一.产品名称：Recombinant Murine IL-4

二.产地：美国Peprotech

三.Catalog Number:AF-214-14

四.Synonyms:BCGF, BCDF, B cell stimulating factor (BSF-1)

五.Description:

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues. Manufactured using all non-animal reagents.

六.AA Sequence:

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

七.Source:E.coli

八.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

九.Biological Activity:

The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg

十.Cross Reactivity:Rat, Pig Intestinal Worm, Mouse, Trout, N/A, Human