Ultrasensitive C-peptide EIA背景介绍：

Mercodia Ultrasensitive C-peptide ELISA provides a method for the quantitative determination of human C-peptide in serum, plasma or urine.

Ultrasensitive C-peptide EIA  Summary and explanation of the test

Qualitative and quantitative evaluation of pancreatic ß-cell function is not onlyof use in the pre and postdiagnostic study of the natural history of diabetesmellitus, but is also relevant in clinical practice as a guide to the correct choiceof treatment. Peripheral insulin levels cannot be used to assess ß-cell functionbecause of a large and variable uptake from the portal circulation into the liver,and because insulin assays cannot distinguish endogenous from exogenousinsulin. Within the pancreatic ß-cell, proinsulin is cleaved into one molecule ofC-peptide and one molecule of insulin. C-peptide is subsequently released intothe circulation at concentrations equimolar to those of insulin. In contrast toinsulin, C-peptide is only minimally extracted by the liver. Peripheral C-peptideconcentrations therefore reflect the secretion of ß-cells more accurately thaninsulin.

Ultrasensitive C-peptide EIA Principle of the procedure

Mercodia Ultrasensitive C-peptide ELISA is a solid phase two-site enzymeimmunoassay. It is based on the direct sandwich technique in which twomonoclonal antibodies are directed against separate antigenic determinantson the C-peptide molecule. During incubation C-peptide in the sample reactswith anti-C-peptide antibodies bound to the microtitration well. After washing,peroxidase conjugated anti-C-peptide antibodies are added. After a secondincubation and a simple washing step, the bound conjugate is detected byreaction with 3,3’,5,5’-tetramethylbenzidine (TMB). The reaction is stopped byadding acid to give a colorimetric endpoint that is read spectrophotometrically.