Porcine Insulin EIA 猪胰岛素检测试剂盒背景介绍：

Mercodia Porcine Insulin ELISA provides a method for the quantitative determination of insulin in porcine serum and plasma.

Porcine Insulin EIA 猪胰岛素检测试剂盒Summary and explanation of the test

Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthesised in the ß-cells of the islets of Langerhans as the precursor,proinsulin, which is processed to form C-peptide and insulin. Both are secretedin equimolar amounts into the portal circulation. The mature insulin moleculecomprises two polypeptide chains, the A chain and the B chain. The two chainsare linked together by two inter-chain disulphide bridges. There is also an intrachain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions. Its principalfunction is to control the uptake and utilisation of glucose in peripheral tissuesvia the glucose transporter. This and other hypoglycaemic activities, such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon, epinephrine (adrenaline), growthhormone and cortisol. Islet cell transplantation has long been considered as a potential cure for type1 diabetes. The shortage of human donors and difficulty in isolating purified isletsfrom adult human pancreas has drawn the attention to porcine pancreaticislets 1-4. Pig and human insulin are structurally similar, and the regulation ofinsulin secretion in pigs resembles humans. Parameters such as insulin secretionare used to investigate the viability of the transplanted islets 4-6.

Porcine Insulin EIA 猪胰岛素检测试剂盒Principle of the procedure

Mercodia Porcine Insulin ELISA is a solid phase two-site enzyme immunoassay.It is based on the direct sandwich technique in which two monoclonal antibodiesare directed against separate antigenic determinants on the insulin molecule.During incubation, insulin in the sample reacts with peroxidase-conjugated antiinsulin antibodies and anti-insulin antibodies bound to the microplate.After a simple washing step that removes unbound enzyme labelled antibody,the bound conjugate is detected by reaction with 3,3´-5,5´-tetramethylbenzidine(TMB). The reaction is stopped by the addition of acid, giving a colorimetricendpoint that can be read spectrophotometrically.