Feline Insulin  EIA 猫胰岛素检测试剂盒背景介绍：

Mercodia Feline Insulin ELISA provides a method for the quantitative determination of insulin levels in feline serum and plasma.

Feline Insulin  EIA 猫胰岛素检测试剂盒Summary and explanation of the test

 Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesised in the ß-cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain and the B chain. The two chains are linked together by two inter-chain disulphide bridges. There is also an intra-chain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration, and the hormone has a number of important metabolic actions. Its principal function is to control the uptake and utilisation of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth hormone and cortisol. Diabetes mellitus is one of the most common edocrine disorder in cats, with a form that closeley resembles human type 2 diabetes (1-2). Its incidence rate among cats appears to be increasing, probably due to an increase in obesity and a decrease in physical activation in the cat population (1-2). Obesity increases the risk for diabetes 3- to 5-fold (2). Diabetes occurs in a wide range of cats, but most cats are over six years of age when diagnosed (1-2). Diabetic cats may go into remission and studies have shown that different insulin tharapy treatments may have an influence on this (3). 

Feline Insulin  EIA 猫胰岛素检测试剂盒Principle of the procedure 

Mercodia Feline Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. During incubation, insulin in the sample reacts with peroxidase-conjugated antiinsulin antibodies and anti-insulin antibodies bound to the microplate. After a simple washing step that removes unbound enzyme labelled antibody, the bound conjugate is detected by reaction with 3,3´-5,5´-tetramethylbenzidine (TMB). The reaction is stopped by the addition of acid, giving a colorimetric endpoint that can be read spectrophotometrically。