Porcine C-peptide EIA 猪C肽检测试剂盒背景介绍:Mercodia Porcine C-peptide ELISA provides a method for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

Porcine C-peptide EIA 猪C肽检测试剂盒SUMMARY AND EXPLANATION OF THE TEST Within the pancreatic ß-cell, proinsulin is cleaved into one molecule of C-peptide and one molecule of insulin. C-peptide is subsequently released into the circulation at concentrations equimolar to those of insulin. In contrast to insulin, C-peptide is only minimally extracted by the liver. Peripheral C-peptide concentrations therefore reflect the secretion of ß-cells more accurately than insulin (1,2). Traditionally C-peptide has been considered to be without biological effects of its own, but in recent years it has been reported that C-peptide treatment may affect renal and nerve dysfunction in type 1 diabetes patients (3). In islet transplantation studies, determination of C-peptide has become an important method to monitor islet function. In xenotransplantation, determination of porcine insulin can usually not be used to monitor islet function due to crossreaction of porcine insulin with insulin from other species. A key parameter of pre-clinical efficacy of islet xenotransplantation is therefore the specific determination of porcine C-peptide responses in the recipient (4). 

Porcine C-peptide EIA 猪C肽检测试剂盒PRINCIPLE OF THE PROCEDURE Mercodia Porcine C-peptide ELISA is a solid phase two-site enzyme immunoassay. It is based on the sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the C-peptide molecule. During incubation, C-peptide in the sample reacts with anti-C-peptide antibodies bound to the microtitration well, clone 4B7-E9. After washing, peroxidase-conjugated anti-C-peptide antibodies clone 5G8-G2, are added and after the second incubation and a simple washing step that removes unbound enzyme labeled antibody, the bound conjugate is detected by reaction with 3,3’-5,5’-tetramethylbenzidine (TMB). The reaction is stoped by the addition of acid, giving a colorimetric endpoint that can be read spectrophotometrically